Spectrophotometric Determination of Copper by using Erythromycin

Research Article

Austin J Anal Pharm Chem. 2023; 10(3): 1165.

Spectrophotometric Determination of Copper by using Erythromycin

Sherif A Kolkaila*; Gehan S Elasala; Alaa E Ali

Department of Chemistry, Faculty of Science, Damanhour University, Egypt

*Corresponding author: Sherif A Kolkaila Department of Chemistry, Faculty of Science, Damanhour University, Egypt. Tel: +201273769704 Email: sherifak@Sci.dmu.edu.eg

Received: November 13, 2023 Accepted: December 23, 2023 Published: December 30, 2023

Abstract

Copper (lI) forms a complex with erythromycin at pH 5 which leads to spectroscopic determination of erythromycin using (UV -Vis). Erythromycin-Copper (lI) complex was stabilized and measured at 404 nm while Beer’s law is valid for the concentration range of 2.5-30 μg.ml-1. Sandell’s sensitivity was 0.284738042 μg.cm-1, However the detection limit was 0.274576 μg.ml-1, and the limit of quantitation was 0.915255 μg.ml-1. Mole ratio method was applied with result (1:1 L:M ) Also Continuous Variation Method was applied.

Keywords: Antibiotic drug; Erythromycin; Erythromycin complex; Determination of copper

Introduction

Erythromycin is very active against Gram-positive and some Gram-negative microorganisms which is used for treatment of respiratory, gastrointestinal, and genital tract infections Figure 1. This work develops an easy method and environmental friendly by using Metal Complexation. A complex between a erythromycin and copper ion is used as a chemo sensing that the complexation leads to a change in colour or fluorescence [1-3]. Transition metal complexes often have spectacular colours caused by electronic transitions by the absorption of light [4,5]. Each wavelength of light has a particular energy associated with it and then the solution of the mixture show the colour of the light it absorbs A charge transfer band entails promotion of electrons from a metal-based orbital into an empty ligand-based orbital (Metal-to-Ligand Charge Transfer or MLCT). UV-Vis spectrometry for its simplicity, versatility, speed and cost-effectiveness. UV-Vis spectrophotometers for environmental monitoring receives much attention once again. The metal ion recognition system is based on the interaction/complexation of the various ligands with heavy metals to form highly coloured complexes. There is a wide choice in commercially available chromo-reagents for heavy metal ion sensing [6,7]. Heavy metals can form a strong coloured complex. The molar absorptivity (e, from Beer-Lambert’s Law), is the parameter to evaluate how strong the heavy metals-indicator complex absorbing light at the given wavelength is. It is the intrinsic property of the absorbing species [8,9]. In this work exploring a method for determination of Copper by using erythromycin.